Abstract

This study evaluates and compares the anti-oxidation protective effects of two liposomal vitamin C formulations LipoVITA^® C (conventional liposomal vitamin C formulation) and LipoVITA^® C PLUS (an advanced modified polymeric surface liposome) against non-liposomal vitamin C (NL- VC). The study employs cell viability assay using human skin fibroblasts exposed to UV radiation module. Vitamin C is a potent but unstable antioxidant; liposomal encapsulation stabilizes it and enhances its efficacy. Human skin fibroblasts were treated with varying concentrations of LipoVITA^® C, LipoVITA^® C PLUS, and NL- Vitamin C (100, 10, 1, 0.1 μg/ml) and subjected to no UV exposure, 3 minutes UV exposure, or 8 minutes UV exposure. Cell viability was assessed using the MTT assay, and statistical analysis using one-way ANOVA, post-hoc tests, and SPSS software revealed highly significant differences between groups (p<0.001). Results showed that LipoVITA^® C PLUS maintained the highest viability across all concentrations and UV exposures. Both liposomal formulations demonstrated concentration-dependent protection and retained over 90% viability at 0.1 μg/ml after 8 minutes of UV exposure, in contrast to the sharp decline in viability with NL- Vitamin C. In conclusion, LipoVITA^® C PLUS proved superior in protecting fibroblasts against UV-induced damage compared to LipoVITA^® C and NL- Vitamin C. The advanced modified polymeric surface liposomes enhanced vitamin C stability and intracellular delivery, thereby reducing cellular oxidation more effectively.