Sarcouncil Journal of Medical Series

Abstract: Background: Antimicrobial resistance among urinary tract pathogens is a growing global threat. Objective: To evaluate antibiotic susceptibility patterns of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa isolated from UTIs in Tay Nguyen University Hospital. Methods: From March to May 2025, 139 positive urine cultures were analyzed. Antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method according to CLSI guidelines. Results: E. coli isolates showed high resistance to Ampicillin (66.7%) and Tetracycline (41.3%), but remained susceptible to Amikacin (95.2%) and Piperacillin/Tazobactam (82.5%). S. aureus exhibited high resistance to Erythromycin (87.0%) but retained sensitivity to Amikacin (97.8%) and Levofloxacin (73.9%). P. aeruginosa isolates were resistant to most tested antibiotics but susceptible to Colistin (92.3%) and Piperacillin/Tazobactam (84.6%). Conclusion: Rising resistance rates highlight the urgent need for routine antimicrobial surveillance and rational antibiotic use in clinical practice.