Abstract

The purpose of the research is the detection of the relationship between anemia in pregnant women in the second trimester of pregnancy and the Virulence of factors for bacterial infections of Escherichia. Coli (E. Coli) by PCR. 150 midstream urine samples were collected from pregnant women with age (16-45) years and also were taken blood samples. The percentage of bacterial Number which was isolated from 105 samples, 44,3 % (n=47) were E. Coli followed by other gram-negative bacteria, 11,3% (n=12), which include Klebsiella pneumonia, and 10,4% (n=11) Pseudomonas aeruginosa. Also was the Number and percentage of 50% (n=35) of the infection group women had anemia, while 100% (n=35) of the non-infected women and non-anemic. Further confirmation was done using by API 20E system and VITEK 2. This study proved the appearance of the cnf1 gene in the E. Coli bacteria, which is infect to pregnant women with anemia, and also showed the toxic role of these genes in tissue damage.) cnf1,16SrRNA).

Keywords

PCR, DNA, E. Coli, VITEK, API, Asymptomatic, Pregnant, Urine, cnf1,16SrRNA.