Abstract

Introduction: Ovarian cancer is the deadliest gynecological malignancy, usually not detected until the late stages. In vitro cell culture used for studies such as the study of interactions of drugs. Dihydromyricetin is a key ingredient in many healthy foods or beverages. It has an excellent safety profile for human. Material-Method: SKOV-3 cells were cultured in RPMI medium with 100 units/mL-100 μg/mL of penicillin-streptomycin and 10% fetal bovine serum in a CO₂ incubator. After incubation for 3 h at 37°C with 5% CO2, the media was removed and 0.1 mL of DMSO was added. The plates were read at 570 nm in the spectrophotometer. The MTT assay was repeated at least three times independently. Following this procedure, RT-PCR was performed. Results: SKOV-3 cells were treated with different concentrations of DHM. DHM was found to be cytotoxic to the SKOV-3 cell line in a time- and concentration-dependent manner. SKOV-3 cells were treated with different concentrations of BEV. Cell viability was decreased at the different concentrations. To evaluate the cytotoxic effects of DHM and BEV together on the SKOV-3 cell line, cells were treated with a combination of IC₅₀ concentrations of DHM and BEV. According to combination results, DHM + BEV treatment was found to be more cytotoxic to SKOV-3 cells than treatment with DHM or BEV alone. Conclusion: In our study, the combined effects of two anti-cancer agents on ovarian cancer cell lines were investigated. It has been determined that the effect of the agents in the combined treatment is greater than that of the agents alone

Keywords

Dihydromyricetin, Bevacizumab, SKOV-3, Anti-cancer effects, MTT, RT-PCR Analysis