In – Vitro Technique to Study the Effect of Sunscreen Cream on the Hair Damage

Abstract

The objective of the present study was to study the effect of sunscreen cream formulation on the damage of hair caused by UV radiation. Hair is one of the vital parts of the body and considered to be a necessary and important component of the overall appeal and beauty of human being. Sunscreen cream was formulated by using Benzophenone-3 and Octyl-methoxy cinnamate as an active ingredients. Evaluation of Sunscreen cream was performed by fluorescence quenching. In this method, Control (without sunscreen) and Test Samples (with sunscreen) of hair were prepared with Curcumin and Alcohol and exposed to short UV radiation in UV chamber. The 12 sets of approximately 100mg of hair sample in Petri dishes were prepared and labeled. Six Petri dishes  (3 Control & 3 Test) were exposed to short wavelength of UV (280-340nm) for respective time span of 1hr, 2hrs and 3hrs, the distance fixed for exposure of hair sample was 20cm from UV lamp. Remaining six samples (3 Control & 3 Test) were exposed to natural sunlight with same time span. At the end of exposure regime, all samples were removed and evaluated for porosity. It has been observed that Control sample showed fluorescence strongly while test sample showed no fluorescence. Hair exposed to natural light led to formation of split strands when treated with 1N NaOH solution. However, hair coated sunscreen cream showed no splitting. Hair (Test) exposed to UV light, led to change in porosity as compared to hair applied with sunscreen cream at the same experimental conditions

Keywords

In Vitro, Sunscreen Cream, Hair Damage