Abstract

The effect study of blood samples that were exposed at different doses of X-ray using the Fluoroscopy technique by electrochemical analysis with the oxidation-reduction properties using the cyclic voltammetric technique to find the oxidative stress of the radiation of X-ray on the blood composition without contrast medium and with contrast, and also, the study focused on the impact of the ascorbic acid as an anti-oxidative reagent on blood without contrast and with contrast medium (iodine solution). In the patient and method that was chosen, 40 patients were divided into 20 females and 20 males, with the range of age from 20 to 40 years. Ten of the patients as control samples without any exposed to the X-ray; the other 30 patients were divided into three groups ten patients exposed with different doses of X-ray (300-600 reem) using fluoroscopy type Shimadzua, type HP/Flowro without contrast medium, ten patients was injected (by IV) with contrast medium (Iodine solution) and exposed with the same different doses of x-ray by fluoroscopy technique to study the effect the radiation on the blood with contrast medium, and the other ten patents were injected with contrast medium and with ascorbic acid to study the impact of ascorbic acid as anti-oxidative reagent on the exposed blood with contrast agent samples. The results illustrated that the blood samples which exposed to various doses of radiation were caused to enhance the oxidation current peak regarding the blood as proportionality with increasing of the radiation dose compared with the control blood sample (not exposed to radiation) and the other blood samples that injected with contrast medium (Iodine solution) were enhanced the oxidation peak more than the control samples and also the samples exposed without contrast medium. The other study was used ascorbic acid, which acts as anti-oxidative stress to the blood with and without a contrast medium. It can  be concluded that the exposed of patients in X-ray caused a high oxidative stress of the blood composition with and without contrast medium, which show more effected with contrast medium, and the ascorbic acid acted as an anti-oxidative reagent that decreased the oxidation current peak when injected the patients with and without contrast medium